The Proline-mediated Reactions of Tobacco Plants, Cultivated Under Salinity
DOI:
https://doi.org/10.29038/2617-4723-2016-337-12-15-19Keywords:
tobacco, cell selection, barium cations, salinity, tolerance, prolineAbstract
The comparative investigation of tobacco plants reactions on lethal salinity pressure and their proline status were the objects of the research.
On selective media with the addition of lethal for cell cultures doses of Ba2+ cations resistant tobacco cell lines occurred. Regenerants R0 and seed progenies R1, R2 were obtained from those lines. Cell lines, R0, R1 and R2 plants challenged the simulated lethal salt stresses. The comparative investigation of proline-mediated reactions of those variants and initial tobacco (pure glicophyte) were elaborated. R0 plants were cultivated in vitro during 35 days on cultural media with the addition of 20,0g/l sea water salts; R1 and R2 plants were tested in water culture with the addition of 25,0 g/l sea water salt during 10 days.
Control and experimentally obtained variants developed different reactions to salt pressure. Control plants eliminated. Experimental variants maintained their metabolism reflected in stable protein biosynthesis. At the same time the free proline levels increased in all genotypes. But in resistant variants these events resulted from amino acid biosynthesis, while in control plants proline appearance was the result of the protein compartments degradation. So data of free proline level are not adequate markers of the plant salt resistance. There is better to estimate the character of proline changes.
References
2. Szabados L. Proline: a multifunctional amino acid / L. Szabados, A. Savoure // Trends Plant Sci. – 2010. – 15. – P. 89–97.
3. Dracup M. Why does in vitro cell selection not improve the salt tolerance of plants? / M. Dracup // Genetic aspects of plant mineral nutrition / Eds. P. J. Randall et al. ; Kluwer Academic Publishers, 1993. – P. 137–142.
4. Деклараційний патент на винахід №44487 А01Н1/04, А01Н4/00 Спосіб відбору стійких до засолення клітинних ліній рослин / Л. Є. Сергєєва, подання 18.04.2001, опубліковано 15.02.2002, бюл. № 2.
5. Николаев Л. А. Биокатализаторы и их модели : учеб. пособие / Л. А. Николаев. – М. : Высш. шк., 1968 – 196 с.
6. Fan L.-M. Identification and characterization the inward K+ channel in the plasma membrane of Brassica pollen protoplasts / L.-M. Fan, W.-H. Wu, H.-Y. Yang // Plant and Cell Physiol. – 1999. – 40, № 8. – P. 859–865.
7. Rubio F. Relative contribution of AtHAK5 and AtAKT1 to K+ uptake in the high-affinity range of concentrations / F. Rubio, M. Nieves-Cordones, F. Aleman, V. Martinez // Physiol. Plant. – 2008. – 134. – P. 598–608.
8. Сергеева Л. Е. Клеточная селекция с ионами тяжёлых металлов для получения генотипов растений с комплексной устойчивостью к абиотическим стрессам / Л. Е. Сергеева. – Киев : ЛОГОС, 2013. – 211 с.
9. Murashige T. A revised medium for rapid growth and bioassays with tobacco tissue culture / T. Murashige, F. Skoog // Physiol. Plant. – 1962. – 15. – P. 473–497.
10. Андрющенко В. К. Модификация метода определения пролина для выявления засухоустойчивых форм рода Lycopersicon Tourn / В. К. Андрющенко, В. В. Саянова, А. А. Жученко и др // Известия Академии наук Молдавской ССР. – 1981. – № 4. – С. 55–60.
11. СтепанченкоН. С. Количественное определение содержания белка // Физиол. растений. – 2011. – 58. – P. 624–630.
12. Stein H. Elevation of free proline and proline-rich protein levels by simultaneous manipulations of proline biosynthesis and degradation in plants / H. Stein, A. Honig, G. Miller et al. // Plant Sci. – 2011. – 181. – P.140–150.
13. Battaglia M. Proline-rich cell wall proteins accumulate in growing regions and phloem tissue in response to water deficit in common bean seedlings / M. Battaglia, R. M. Solorzano, M. Hernandez et al. // Planta. – 2007. – 225. – P. 1121–1133.
