Tissue Respiration in the Liver and Bile Secretion at the Action of L-cysteine

Authors

  • Yliya Levadianska Taras Shevchenko National University of Kyiv
  • Evdokiya Reshetnik Taras Shevchenko National University of Kyiv
  • Stanislav Veselsky Taras Shevchenko National University of Kyiv
  • Petro Yanchuk Taras Shevchenko National University of Kyiv

DOI:

https://doi.org/10.29038/2617-4723-2018-377-108-114

Keywords:

polarography, oxygen tension, oxygen consumption, thin-layer chromatography, bile acids, lipid bile

Abstract

The specific function of the liver is bile secretion, the synthesis and transport of certain organic components which are directly related to the level of activity of tissue respiration in the gland. Its modulator may be an amino acid L-cysteine, which is a precursor of a gas transmitter of hydrogen sulfide (H2S), which can affect the supply of oxygen to the liver with blood and metabolic processes in it.

Purpose. The aim of the present study was to investigate the effect of L-cysteine on the level of oxygen in the liver tissue and to find out the relation of this indicator with the dynamics of changes in concentrations of bile acids and lipids in bile in rats.

Methods. The level of oxygen tension (pO2) in the liver tissue was recorded by the polarographic method. The coefficient of oxygen consumption by the liver (K) was calculated by the curve of falling pO2 in the gland during the occlusion of its blood vessels. Concentrations of bile acids and lipids of bile were determined by thin layer chromatography.

Results. Our results indicate that administration of L-cysteine at a dose of 20 mg/kg body weight causes the maximum fall in pO2 in the liver parenchyma by 46,8 % (p <0,01) from baseline. This may indicate the activation of processes associated with the consumption of oxygen in the gland, which led to the drop of pO2. Along with this, there was an increase in the level of tauroconjugates in the bile, in particular, the concentration of taurocholic acid by 7,3 % (p <0,05) and the mixture of taurodezoxychole and taurohenodeoxycholic acids by 17,9 % (p <0,05) relative to the initial equal. At the same time, the content of free fatty acids and triglycerides in the liver secretion decreased by 12,3 % and 18,5 % (p <0,05) respectively in the fifth half-hourly sample relative to the baseline level. The concentration of phospholipids decreased in the sixth half-hourly sample by 12,5 % relative to the baseline level.

Conclusion. Thus, L-cysteine causes an increase in oxygen-dependent synthetic processes in the liver, in particular, associated with mitochondrial poly-enzymes bile acid biosynthesis systems and oxidation of some lipid fractions of bile with simultaneous activation in hepatocytes of tissue respiration. The level of oxygen supply in the gland decreases.

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Published

2018-06-26

How to Cite

Levadianska, Y., Reshetnik, E., Veselsky, S., & Yanchuk, P. (2018). Tissue Respiration in the Liver and Bile Secretion at the Action of L-cysteine. Notes in Current Biology, (4(377), 108–114. https://doi.org/10.29038/2617-4723-2018-377-108-114