Effect of Intravenous Treatment of Silver Nanoparticles on Oocytes and Cells of their Follicular Environment under Conditions of Experimental Glomerulonephritis
DOI:
https://doi.org/10.29038/2617-4723-2018-377-92-99Keywords:
female reproduction, functional state of the ovary, nanotechnology, nanomedicineAbstract
Nowadays, biomedical nanotechnology and nano medicine develops rapidly in the search for new drugs. Among them drugs based on silver nanoparticles (AgNPs) occupy the leading position. Studies that assess the effect of different doses and the multiplicity of the introduction of various sizes of AgNPs on reproduction of female animals gain topicality. Such studies will provide new data that will contribute to a fuller understanding of AgNPs mechanisms of action in the laboratory as well as provide a successful transition of silver nanotechnology into the clinics. Hence, the effect of AgNPs on mammalian cells and tissues requires further research.
The aim of the given study was to estimate the effect of intravenous treatment of silver nanoparticles on the functional state of the ovary in mice, namely oocytes (ovarian and meiotic maturation) and cells of their follicular environment (number of live, with morphological signs of apoptotic and necrotic death), under conditions of experimental glomerulonephritis.
Experimental glomerulonephritis in mice was achieved by immunization of white laboratory mice of the first generation with a kidney antigen suspension derived from a parent. The treatment was carried out the following way: kidney antigen Suspension - intraperitoneal three times 1 time per day; the procedure was repeated in 3 weeks, one time intraperitoneally with the same dose (10 mkL of suspension per 10 grams of body weight of the animal). Silver nanoparticles (AgNPs, 30 nm) – intravenous three times: 1 time per day for 1 hour before immunization of animals with suspension of kidney antigen; as well as in 3 weeks once with the same dose (2 mg/kg).
Thus, the number of oocytes that resume meiosis in vitro from animals under experimental glomerulonephritis decreases comparing to the numbers in control animals; no inhibition of meiotic resumeption of oocytes of animals was established under conditions of AgNPs treatment; the AgNPs treatment under conditions of experimental glomerulonephritis increases the number of oocytes that resume meiosis in vitro compared with such values in the control and under conditions of experimental glomerulonephritis as well as the number of living cells increases and the number of cells with morphological signs of apoptotic death decreases in the follicular environment of oocytes.
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