The Morphological Features of Anatomy Arterial of Jaw-oral Area in Male Rats in Normal and Influence of Different Types of Inflammatory Reactions Under the Provided to Experimental Gastroduodenitis

Abstract

Introduction. By now the features of jaw-oral area blood vessels structural and dimensional reorganization according to inflammation in upper portion of gastrointestinal tract are not studied well enough, especially in the context of different correspondence to the inflammation. Aim of the research. To define dimensional and morphometrical specifics of rearrangement of major blood vessels of the jaw-oral area in white male rats due to experimental gastroduodenitis among rats with different types of inflammatory reactions. Materials and methods.The research was conducted on 24 white male rats of 180–200 grams each, that were divided into 4 groups: 1 group – intact animals; 2 group – animals with gastroduodenitis with normoergic type of inflammatory reaction; 3 group – animals with gastroduodenitis with hypoergic type of reaction; 4 group – animals with gastroduodenitis with hyperergic one. The acute gastroduodenitis was modelled by probe intragastric input of 0, 25 ml of 10 % ethanol and 0,5 ml of 1,25 % hydrochloric acid with 5 minutes interval. The following manipulation was conducted once per day 7 days in a row. Hypoergic type of inflammatory reaction was modelled by intramuscular injection of cyclophosphamide (OAS «Kyivmedpreparat», Ukraine) in a dose 10 mg/kg of body weight 3 days before and each day of experiment. Hyperergic type of inflammatory reaction was modelled by intramuscular injection of pyrogenalum (NRIEM named after M. F. Gamaleya RAMS, Russia) with 0,9 % NaCl – 5 minimal doses during 1 day before and each day of experiment. The contrast X-ray angiograms of rats’ craniums were studied, analyzing the state of vessels tees in which were measured diameters of the main trunk and subsidiary branches, angles of deviation, coefficients of asymmetry and branching. Results and discussion. In experimental gastroduodenitis among white rats capacitive indexes of arterial blood flow of jaw-oral area increase. This can be proved by increasing diameters of blood vessels tees comparing to such in group of control, the most significant changes were observed among rats with hyperergic type of reaction. There could also be observed branching alteration and changing of branching index, that increased at bigger arteries, at smaller arteries with normoergic type of inflammatory reaction almost had not changed ( difference –1,59 %), with hypoergic decreased on 10,59 % and with hyperergic type of inflammatory reaction increased on 6,69 %. Such dynamics can be considered as various ways of stereo metric rearrangement of small arteries and microcirculatory blood flow. Comparing angles of deviation of subsidiary branches of the tees the steady increase among all groups, except branches of first line among rats with hyperergic type of reaction, can noted. The structural and functional changes of gastrointestinal tract among white rats due to experimental gastroduodenitis are followed by remodeling of the arteries of jaw-oral area arteries remodeling.  Such remodeling occurs because of blood vessels capacity changing as well as dimensional features, which is shown in dynamics of asymmetry index and angles of branching changes. Intensity of remodeling depends on type of inflammatory reaction – the most significant it was among rats with hyperergic type of inflammatory reaction. Among animals with hypoergic type of inflammatory reaction remodeling occurred mostly because of dimensional rearrangement of arterial tees.