Peculiarities of protein synthesis in Pleurotus ostreatus mycelium for laser irradiation
DOI:
https://doi.org/10.29038/2617-4723-2020-390-2-25-30Keywords:
Pleurotus ostreatus, biomass, protein, surface cultivation, photoactivationAbstract
Features of protein synthesis in the mycelium of Pleurotus ostreatus under the action of laser irradiation. Kateryna Reshetnyk.
Basidiomycetes are valuable biological objects that are used to produce biologically active substances. The aim of our work was to study the effect of laser irradiation on biomass accumulation and protein synthesis processes in Pleurotus ostreatus mycelium. Strains of the fungus P. ostreatus from the Collection of cultures of basidiomycetes of the Department of Botany and Ecology of Vasyl' Stus Donetsk National Universitywere used for the study. Irradiation of the inoculum with a size of about 55 mm (always of the same density and age) was performed before sowing using LED lasers: BRP-3010-5 (wavelength 635 nm), BBP-3010-5 (wavelength 405 nm) and BGP-3010–5 (wavelength 532 nm). The power of each laser was 100 mW. The irradiation energy in all variants of the experiment was 51.1 mJ/cm2. The level of biomass accumulation was determined by weight method, drying the mycelium to constant weight at a temperature of (105 ± 1)°C. In order to determine the amount of protein used the method of biuret reaction. Our research results indicate the effect of laser irradiation on the stimulation of growth and biosynthetic processes of P. ostreatus. In particular, for P. ostreatus, green light irradiation with a wavelength of 532 nm was the most effective. Under the action of this irradiation regime, the best reaction in response to light was observed for strain P-192 – the amount of biomass increased by 71.4%. An increase in the amount of protein in the mycelium of P. ostreatus was found under the action of laser irradiation. For strain P-192 of the fungus P. ostreatus, the total protein content increased by 36.3% in accordance with the
control over the effects of green light irradiation (532 nm). Our results show the prospects of using laser irradiation with light in the green range for targeted regulation of protein synthesis and biomass of mycelium of the fungus P. ostreatus.
References
2. Alekseenko, E.N.; Polishko, T.M.; Vinnikov, A.I. Harchova, likuvalna ta ekologichna tsinnist gribiv Pleurotus ostreatus [Food, medicinal and ecological value of fungi Pleurotus ostreatus]. Bulletin of Dnipropetrovsk University. Biology. Ecology. 2010, 18 (1), pр. 3–9. (in Ukrainian)
3. Velichko, T.O.; Zubareva, I.M.; Mitina, N.B.; Tkalya, O.I.; Shatalin, D.B. Optymizatsiia pozhyvnykh seredovyshch dlia kultyvuvannia Pleurotus ostreatus [Optimization of nutrient media for cultivation of Pleurotus ostreatus]. Scientific works of the Odessa National Academy of Food Technologies. 2011, 40 (2), pp. 165–167. (in Ukrainian)
4. Friedl, M. A.; Schmoll, M.; Kubicek, C. P.; Druzhinina, I. S. Photostimulation of Hypocrea atroviridis growth occurs due to a crosstalk of carbon metabolism, blue light receptors and response to oxidative stress. Microbiology. 2008, 154, рр. 1229–1241.
5. Tisch, D.; Kubicek, C. P.; Schmoll M. Crossroads between light response and nutrient signalling: ENV1 and PhLP1 act as mutual regulatory pair in Trichoderma reese. BMC Genomics. 2014, 15, рр. 425–438.
6. Kamada, T.; Sano, H.; Nakazawa, T.; Nakahori, K. Regulation of fruiting body photomorphogenesis in Coprinopsis cinerea. Fungal Genetics and Biology. 2010, 11, рр. 917 – 921.
7. Poedinok, N. L. Energoefektivni sistemi shtuchnogo osvitlennya u tehnologiyah viroschuvannya yistivnih ta likarskih gribiv [Energy-efficient artificial lighting systems in technologies for growing edible and medicinal mushrooms]. Science and innovation. 2013, 9(3), pp. 46-59. (in Ukrainian)
8. Dudka, I. A.; Vasser, S. P.; Ellanskaya, I. A. i dr. Metodyi eksperimentalnoy mikologii. [Experimental mycology methods]. Directory. К: Nauk. Dumka. 1982. 561 р. (in Russian)
9. Prysedskyi, Yu. H. Statystychna obrobka rezultativ biolohichnykh eksperymentiv. [Statistical processing of the results of biological experiments]. Donetsk: Cassiopeia. 1999. 210 p. (in Ukrainian)
10. Prysedskyi, Yu. H. Paket prohram dlia provedennia statystychnoi obrobky rezultativ biolohichnykh eksperymentiv. [A package of programs for statistical processing of the results of biological experiments]. Donetsk: DonNU. 2005. 84 p. (in
Ukrainian)
11. Poedinok, N. L. Biotehnologicheskie osnovyi intensifikatsii kultivirovaniya s'edobnyih i lekarstvennyih makromitsetov s pomoschyu sveta nizkoy intensivnosti [Biotechnological bases of intensification of cultivation of edible and medicinal
macromycetes using low intensity light]: dis. ... Dr. Biol. Sciences: 03.00.20. Kiev, 2015.387 p. (in Russian)
12. Hillenkamp, F. Interaction between laser radiation and biological systems. Lasers in Biology and Medicine. New York; London, 1987, рр. 36–75.
13. Bykov, A.V. Lazernaya elektrodinamika, elementarnyie i kogerentnyie protsessyi pri vzaimodeystvii lazernogo izlucheniya s veschestvom. [Laser electrodynamics, elementary and coherent processes in the interaction of laser radiation with matter]. Moscow: Fizmatlit, 2006, 384 p. (in Russian)
