Influence of laser irradiation on the accumulation of biomass and exopolysaccharides of the Schizophyllum commune Fr.
DOI:
https://doi.org/10.29038/2617-4723-2020-1-389-25-30Keywords:
Schizophyllum commune, schizophilan, surface cultivation, photoactivationAbstract
The influence of laser irradiation on the accumulation of biomass and exopolysaccharides of some strains of S. commune has been studied. To obtain the inoculum of mycelium of strains S.c.-03, S.c.-01, and S.c.-02 of S.commune was cultured on agar glucose-peptone medium. In the study of the effect of laser irradiation of the mycelium in a surface culture used glucose-peptone liquid nutrient medium. The number of exopolysaccharides was determined by the phenol-sulfur method. The most efficient in terms of biomass accumulation and productivity of exopolysaccharide synthesis was the strain S.c.-03 of S.commune – under the action of laser irradiation of mycelium with red light (wavelength 635 nm) mycelium biomass increased by 75.3% and the number of exopolysaccharides increased by 70%.
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3. Arboleda, Valencia J.W.; Valencia Jiménez A.; Gonçalves de Siqueira F.; Dussan, Medina K.; Restrepo, Franco G.M.; Filho, E.X.F.; Grossi‐de‐Sa M.F. Holocellulase activity from Schizophyllum commune grown on bamboo: A comparison with different substrates. Current Microbiology. 2011, 63(6), рр 581–587.
4. Singh, M.K.; Kumar, M.; Thakur, I.S. Proteomic characterization and schizophyllan production by Schizophyllum commune ISTL04 cultured on Leucaena leucocephala wood under submerged fermentation. Bioresource Technology. 2017, 236, рр 29–36.
5. Horisawa, S.; Ando, H.; Ariga, O.; Sakuma, Y. Direct ethanol production from cellulosic materials by consolidated biological processing using the wood rot fungus Schizophyllum commune. Bioresource Technology. 2015, 197, рр 37–41.
6. Ooi, V. A review of pharmacological activities of mushroom polysaccharides. Int. J. Med. Mushr. 1999, Vol. 3, рр 361–394.
7. Rice, P.J.; Lockhart, B.E.; Barker, L.A.; Adams, E.L.; Ensley, H.E.; Williams, D.L. Pharmacokinetics of fungal (1–3)-β-D-glucans following intravenous administration in rats. Int. Immunopharmacol. 2004, 4(9), рр 1209–1215.
8. Suzuki, T.; Tsuzuki, A.; Ohno, N.; Ohshima,Y.; Adachi, Y.; Yadomae, T. Synergistic action of beta-glucan and platelets on interleukin-8 production by human peripheral blood leukocytes. Biol Pharm Bull. 2002, 25(1), рр 140–144.
9. Linovytska, V. M.; Bukhalo, A. S.; Shved, O. M.; Duhan, O. M. Stvorennia biotekhnolohii otrymannia ekzopolisakharydiv na osnovi hlybynoho kultyvuvannia vyshchoho bazydiomitsetu Schizophyllum commune [Biotechnology development of exopolisaccharides on the basis of glybinic cultivation of edible basidiomycetu Schizophyllum commune] Bulletin of the National University «Lvivska Politekhnika», series of Chemistry, technology of speech and storing. 2011, 700, s 161–172. (In Ukrainian)
10. Dudka, I. A.; Vasser, S. P.; Ellanskaya, I. A. i dr. Metodyi eksperimentalnoy mikologii. [Experimental mycology methods]. Directory. Nauk. Dumka: Kyi'v, 1982; 561 р. (In Russian)
11. Varbanets, L. D.; Zdorovenko, G. M.; Knirel, Yu. A. Metodyi issledovaniya endotoksinov. [Endotoxin research methods]. Nauk. Dumka: Kyi'v, 2006; 238 p. (In Russian)
12. Prysedskyiб Yu. H. Statystychna obrobka rezultativ biolohichnykh eksperymentiv. [Statistical processing of the results of biological experiments]. Cassiopeia: Donetsk, 1999; 210 p. (In Ukrainian)
13. Prysedskyi, Yu. H. Paket prohram dlia provedennia statystychnoi obrobky rezultativ biolohichnykh eksperymentiv. [A package of programs for statistical processing of the results of biological experiments]. DonNU: Donetsk, 2005; 84 p. (In Ukrainian)
14. Smirnou, D.; Krcmar, M.; Prochazkova, E. Chitin-Glucan complex production by Schizophyllum commune submerged cultivation. Polish J. Microbiol. 2011, 60, рр 223–228.
15. Kama, I.S.M.; Halim, K.H.A.; Rashid, N.M.N.; Mel, M. Optimization of media for mass production of Schizophyllum commune using response surface methodology (RSM). Malaysian International Conference on Trends in Bioprocess Engineering (MICOTriBE). 2012, рр 1–8.
16. Rau, U. Glucans secreted by fungi. Turkish Electronic Journal of Biotechnology. 2004, 2, рр 30–36.
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Published
2020-09-15
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Botany
How to Cite
Influence of laser irradiation on the accumulation of biomass and exopolysaccharides of the Schizophyllum commune Fr. (2020). Notes in Current Biology, 1(389). https://doi.org/10.29038/2617-4723-2020-1-389-25-30
