There is the wheat biotechnology. Free proline levels during early stages of fall wheat genotypes growth as the evaluation marker heat tolerance
DOI:
https://doi.org/10.29038/2617-4723-2019-388-4-17-27Keywords:
fall wheat, dry grain heat, germination, proline, t0-toleranceAbstract
Modern biotechnologies of wheat are directed towards the acceleration of selection process. The obtaining of stress tolerant forms is especial object of this program. The reliable tolerance marker can provide a successful result. Free proline level is the established indicator of plant stress tolerance.
The post t0-stress viability of three wheat genotypes there was evaluated. Genotypes differed for their heat tolerance (genotype 1 < genotype 2 < genotype 3). Dry grains were incubated in hot water (t0 560 C during 20 minutes) and then were germinated under normal conditions. Control variants were germinated on moist paper. Free proline levels were measured in young shoots on the 1-st and the 10-th days after t0-treament.
On the 10-th day after t0-treatment the variability among genotypes in shoots numbers appeared. 15,8 27,0 і 57,0% of 1, 2, 3 genotypes were counted. The shoot lengths varied too: genotype 1 – 10-15cm; genotype 2 – 12-18cm; genotype3 – 18-20 cm. At the first day after temperature treatment the free proline levels in tested shoots were higher than those parameters of control variants. The genotypes were disposed: genotype 1< genotype 2 < genotype 3. At the 10-th day free proline contents decreased and corresponded to control data. There were no differences among genotypes. Endogenic proline was a result of grain proteins hydrolysis. The proline decrease was probably due to it using for young plant organs formation and growth. This event shows the degree of metabolism stress inhibition. The free proline levels in wheat shoots on early stages of plant germination may be the indicator of genotype heat tolerance Such approach can essentially accelerate wheat selection.
References
2. Terletskaya N. V. Nespetsyficheskie reaktsyi zernovykh zlakov na abioticheskie stressy in vivo i in vitro [Unspecific reactions of grain cereals to abiotic stresses in vivo and in vitro] Almaty: 2012 – 208p. (in Russian)
3. Hasegawa, P.M., Bressan, R.A., Zhu, J.K. & Bohnert, H.J. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol. 2000, 51, pp. 463-499.
4. Szabados L., Savoure A. Proline: a multifunctional amino acid Trends Plant Sci. 2010, 15. pp.89-97.
5. Mohammadkhani N., Heidari R. Drought-induced accumulation of soluble sugars and proline in two maize varieties. World App. Sci. J. 2008, 3(3), pp.448-453.
6. Kaur .G Asthir B. Proline: a key player in plant abiotic stress tolerance. Biol. Plant. 2015, 59 (4), pp.609-619. Doi: https://doi.org/10/1007/s10535-015-0549-3
7. Sergeeva L.E., Mykhalska S.I., Komisarenko A.G. Sovremennye biotehnologii povysheniya ustoichevosti rastenii k osmoticheskim stressam [Modern biothechnologies towards increase plant osmotic stresses tolerance] Kiev, 2019, 164p (in Russian)
8. Cha-Um S., Takabe T., Kirdmanee C. Ion contents, relative electrolyte leakage, proline accumulation, photosynthetic abilities and growth characters of oil palm seedlings in response to salt stress Pak. J. Bot. 2010, 42(3), pp.2191-2120.
9. Kamran M., Shahbaz M., Ashraf M., Akram N.A. Alleviation of drought-induced adverse effects in springwheat (Triticum aestivum L.) using proline as a presowing seed treatment Pak. J. Bot. 2009, 41, pp.621-632.
10. Kaur D., Grewal S.K., Kaur J., Singh S. Differential proline metabolism in vegetative and reproductive tissues determine drought tolerance in chickpea Biol. Plant. 2017, 61(2), pp.359-366.// Doi.org/10.1007/s10535-016-0695-2
11. Udovenko G.V. (Ed) Diagnostika ustoichivosti rastenii k stressovym vozdeistviyam [Detection of plant stress resistance] Leningrad: VIR, 1988, pp.128-154 (in Russian).
12. Andriushchenko V.K., Sayanova V.V., Zhuchenko A.A., Diyachenko N.I., Chilikina L.A., Drozdov V.V., Korochkina S.K., Cherep G.I., Medvedev V.V., Niutin Yu.I. Modifikatsiya metoda opredeleniya proline dla vyyavleniya zasukhoustoichivukh form roda Lycopersicon Tourn [The modification of proline estimation method for detection drought tolerant forms of genus Lycopersicon Tourn] // Izv. Akad. Nauk Mold. SSR 1981, №4, pp.55-60 (in Russian).
13. Dospekhov, B.A. Metodika polevogo opyta (s osnovami statisticheskoi obranotki resultatov issledovanii) [Fild experiment methodology (with the basic statistical analysis of research results), 5th edition, complete, , Agtropromizdat: Moscow 1985, 351 p. (in Russian).
14. Stein H., Honig A., Miller G., Erster O., Eilerberg H., Csonka L.N. Elevation of free proline-rich protein levels by simultaneous manipulation of proline biosynthesis and degradation in plants. Plant Sci. 2011, 21. pp. 140-150.
15. Žilić S., Barać M., Pešić M., Dodig D., Ignjatović-Micić D. Characterization of proteins from grain of different bread and durum wheat genotypes Int. J. Mol. Sci. 2011, 12, pp.5878-5894;// doi: 10.3390/ijms 12095878
16. Chacraborty K., Khan K. Biochemical and breadmaking properties of wheat protein components. Compositional differences revealed through quantitation and polyacrylamide gel electrophoresis of protein fractions from various isolation procedures Cereal Chem. 1988, 65, pp.333-340.
