There is the wheat biotechnology. Free proline levels during early stages of fall wheat genotypes growth as the evaluation marker heat tolerance
Keywords:fall wheat, dry grain heat, germination, proline, t0-tolerance
Modern biotechnologies of wheat are directed towards the acceleration of selection process. The obtaining of stress tolerant forms is especial object of this program. The reliable tolerance marker can provide a successful result. Free proline level is the established indicator of plant stress tolerance.
The post t0-stress viability of three wheat genotypes there was evaluated. Genotypes differed for their heat tolerance (genotype 1 < genotype 2 < genotype 3). Dry grains were incubated in hot water (t0 560 C during 20 minutes) and then were germinated under normal conditions. Control variants were germinated on moist paper. Free proline levels were measured in young shoots on the 1-st and the 10-th days after t0-treament.
On the 10-th day after t0-treatment the variability among genotypes in shoots numbers appeared. 15,8 27,0 і 57,0% of 1, 2, 3 genotypes were counted. The shoot lengths varied too: genotype 1 – 10-15cm; genotype 2 – 12-18cm; genotype3 – 18-20 cm. At the first day after temperature treatment the free proline levels in tested shoots were higher than those parameters of control variants. The genotypes were disposed: genotype 1< genotype 2 < genotype 3. At the 10-th day free proline contents decreased and corresponded to control data. There were no differences among genotypes. Endogenic proline was a result of grain proteins hydrolysis. The proline decrease was probably due to it using for young plant organs formation and growth. This event shows the degree of metabolism stress inhibition. The free proline levels in wheat shoots on early stages of plant germination may be the indicator of genotype heat tolerance Such approach can essentially accelerate wheat selection.
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