Influence of N-Acetylcysteine on Movement Activity of Hemipar-kinsonian Rats Induced by Dopamine Receptor Agonist Injection
DOI:
https://doi.org/10.29038/2617-4723-2019-387-173-178Keywords:
N-acetylcysteine, antioxidant, hemiparkinsonism, muscular activity, muscle fatigueAbstract
The muscle contraction during labor activity is accompanied by motor and postural disorders as a results of their fatigue or chronic pain. It is known, that in the process of muscle fatigue development the metabolism is disturbed, products of incomplete oxidation of oxygen – peroxide, free radicals, oxygen ions – are formed. The cells protection from such damage is provided by the antioxidant system. In the field of sport physiology, in the study of muscle fatigue, exogenous antioxidant such as N-acetylcysteine (NAC) is widely used, which accelerates the muscles recovery process after their fatigue. The aim of this study was to detect the preventive effect of a previous injection of NAC on the development of muscle fatigue in a non-anesthetized animal with experimental hemiparkinsonism during prolonged circulatory movements induced by the injection of apomorphine (AM).
The studies were conducted on the Wistar-Kyoto line rats, which caused a one-sided destruction of the dopaminergic (DA) upright system of the brain by injection of 8 μg 6-hydroxidophan dissolved in 4 μl of physiological solution with the addition of 0,1 % of ascorbic acid, which inhibits the oxidation of neurotoxin.
Animals behavioral reactions to the dopaminomimetics injection were an indirect test for the DA-neurons degeneration level in the middle brain. After seven days of 6-hydroxidophanum administration, the animals were divided into the groups: 1 control animals, which caused intense circulatory movements by injection of apomorphine (0,5 mg / kg) (n = 6); 2–rats, which were injected 0.5 ml of physiological solution (n = 6) one hour prior to the injection of AM; 3–animals that were injected with an N-acetylcysteine solution (150 mg / kg) (n = 6) an hour before the injection of AM.
By comparing behavioral tests in rats in three groups, it can be assumed that the decrease in the number of rotation in the control group rats and the animals with the prior injection of the physiological solution was not due to the finish of the apomorphine action, but due to the development of muscle fatigue during prolonged circulatory movements. At the same time, in the rats of the third group, after the application of NAC, there was no decrease in the average number of rotation. This may indicate the activation of the antioxidant defense activity in response to prolonged muscular activity, and the NAC can be considered as a powerful activator of protective mechanisms which reduce the fatigue of skeletal muscle.
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