Effect of L-Glutamic Acid and Pyridoxine on Immunological and Hematological Parameters under the Action of Epinephrine- Induced Stress in Rats
Keywords:L-glutamic acid, cells immunity, T-lymphocytes, erythrocytes, leucocytes
Glutamic acid (L-Glu) is the most abundant and universal amino acid in the body. In almost every cell, L-Glu can be used as a substrate for nucleotide synthesis, NADPH, antioxidants and many other biosynthetic pathways involved in the maintenance of cellular integrity. The L-Glu cytoprotective and antioxidant properties may be extremely important in oxidative stress conditions. Searching for substances which would contribute to faster adaptation of the body under oxidative stress conditions is of current importance . The purpose of the study was to investigate the effect of L-Glu alone and in combination with pyridoxine (L Glu+Pyr) under the influence of epinephrine-induced stress in rats. To mitigate the effect of oxidative stress in our studies, we investigated the impact of the above substances on T- and B-cell immunity, the total number of erythrocytes and leukocytes and phagocytic activity of neutrophils.
It has been shown that under the action of epinephrine and the additional administration of L-Glu and LGlu+ Pyr, the receptor apparatus of T lymphocytes has changed. It was found that intraperitoneal administration of epinephrine in the first experimental group of animals without additional application of L-Glu and L-Glu+Pyr resulted in an increase (p<0,05) in the ratio of T-helper to the cytotoxic T-lymphocytes compared to control group of animal. The decrease in the number of T lymphocytes with zero (0) and average (6–10) receptors density (p<0,05), the number of T-suppressors (р<0,05) in the first animal research groups in comparison to the control group of animals was observed. The additional administration of L-Glu and L-Glu + Pyr has an effect on the T-cell immunity, specifically on the number of T-lymphocytes by increasing the body's defenses, which may be evidenced by the absence of changes compared to control.
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