Features of the Cellular Composition of the Mammals Splenic Pulp
DOI:
https://doi.org/10.29038/2617-4723-2018-381-49-55Keywords:
spleen, mammals, lymphocyte, macrophage, destroyed cells, morphologyAbstract
Studying the cellular composition of the spleen of animals is important for assessing the morphofunctional state of the organ.The purpose of our study was to identify the cellular composition of the parenchyma of the spleen of mammals and the density of placement of lymphocytes in it. The spleen for research was selected from animals that were chosen according of morphofunctional maturity of the organ: from sheep, pig, horse, cattle, rabbit. The sections of the organ were fixed with 10–12 % formalin, washed, dehydrated, poured paraffin, stained with hematoxylin Ehrlich` and eosin and by Bracht methods; a splenic piece fixed in a 2,5 % solution of glutaraldehyde, dehydrated, poured into a mixture of epon-araldit for electron microscopic study. The coefficient of a settling of lymphocytes of white and red pulp was calculated. The cell composition of the white pulp of the mammal`s spleen was represented by lymphocytes, macrophages, which were accounted for up to 90 % of the total cell mass, to 1,3 % of the cells with signs of destruction. The small lymphocytes were dominated, in a significant number of cases there were middle lymphocytes and the smallest number of large lymphocytes. The largest number of small lymphocytes is localized in the white pulp of the rabbit spleen (68,12 %) in the studied animals, the smallest number – in the horse spleen (50,87 %). The medium lymphocytes are found in lesser numbers: from 16,70 % (cattle) to 24,19 % (horse). The smallest number is detected in large lymphocytes and macrophages in cells conditions, this quantity did not exceed 1,5 % of the total number of cells in the splenic white pulp. The largest macrophages were detected in the white pulp of the rabbit`s spleen (1,53 %), large lymphocytes – in sheep (1,43 %). The lymphocyte population coefficient of the splenic pulp in the rabbit is 1,41; in sheep – 1,32; in pigs – 1,39; in horse – 1,52 and 1,43 – in cattle. We consider that study of cytopulations of the spleen of other representatives of vertebrates in the comparative-evolutionary aspect is perspective studies.
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