Sensitivity to Аntibacterial Drugs of Facultative Anaerobic Microorganisms Isolated From Patients With Acute Necrotic Pancreatitis
DOI:
https://doi.org/10.29038/2617-4723-2018-381-5-10Keywords:
acute necrotic pancreatitis, sensitivity, microbiological diagnosis, bacteriaAbstract
Study of the species composition of facultative-anaerobic microorganisms in patients with acute necrotic pancreatitis and their sensitivity to antimicrobial drugs. Identification and sensitivity to antibiotics, by serial dilutions, in microorganisms were carried out on a microbiological analyzer VITEK 2 compact 15 (France). Determination of the sensitivity of isolated microorganisms' cultures to antibacterial preparations was also carried out using the disc diffusion method. Highlighted from manure strains tested for sensitivity to the following antibiotics: gentamicin, linezolid, ciprofloxacin, amikacin, netilmitsyn, aztreonam, fosfomycin, teicoplanin, vancomycin, tiyhetsyklin, levofloxacin, tetracycline, ofloxacin, imipenem, meropenem, piperacillin / tazobactam, ampicillin, ceftriaxone, cefpodoxime, doxycycline, colistin, cefepime, ceftazidime, tobramycin.
In the study of pancreatic manure obtained during an endoscopic examination and during and after surgical interventions and during the rehabilitation of the abdominal cavity in patients with acute necrotic pancreatitis, a variety of facultative anaerobic microflora has been isolated. In the endoscopic study, gram-positive flora was excreted in 27,89 % of cases, during and after surgical interventions and abdominal sanation in 27,36 % of cases. In the endoscopic study, gram-negative flora was excreted in 21,05 % of cases, during and after surgical interventions and in abdominal sanation in 23,7 % of cases.
Due to the fact that in the protocols for the provision of medical care in the section «Diagnostic program» there is no microbiological research, and determination of sensitivity to antibiotics in isolated strains, and to some recommended antibiotics the right to choose an antibiotic belongs to a physician, to increase the experience regarding the data of resistance of microorganisms will help to improve the treatment of purulent necrotic pancreatitis. The conducted studies confirm the proliferation of multiple antibiotic resistance among isolated strains. It was established that in the microflora isolated from patients with NNP, the highest percentage of susceptible strains was, teykoplanina, cefamandol, colistin, linzolid, vancomycin, taigecycline. The obtained results of the research can be used in the future to improve the treatment tactics of patients with acute necrotic pancreatitis.
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