Influence of Microwave Radiation on Antioxidant System in the Tissues of Embryos and Daily Quail

Authors

  • Оlexandr Tsybulin Bila Tserkva National Agrarian University

DOI:

https://doi.org/10.29038/2617-4723-2016-337-12-126-131

Keywords:

microwave radiation, mobile phone, embryogenesis, oxidative stress, antioxidants

Abstract

Long-term exposure of humans to low intensity radiofrequency electromagnetic radiation (RF-EMR) leads to a statistically significant increase in tumor incidence. Mechanisms of such the effects are unclear, but features of oxidative stress in living cells under RF-EMR exposure were previously reported. The aim of our study was to investigate the effect of microwave radiation on the state of the antioxidant system in the tissues of embryo and daily quail. Embryos of Japanese quails were exposed in ovo to extremely low intensity RF-EMR of GSM 900 MHz (14 μW/cm2) during 158–456 h discontinuously (48 c – ON, 12 c – OFF) before and in the initial stages of development. Significant oxidative effect of microwave radiation on the model of quail embryos was demonstrated. The effect was manifested in increased level of lipid peroxidation and decreased activity of key enzymes of antioxidant system.

References

1. Maes W. [Stress caused by electromagnetic fields and radiation] / W. Maes. – Neubeuern, Germany : IBN, 2005. – Р. 602
2. [Elektronik resourse]. – Mode of access : http://www.itu.int/en/itu-d/statistics/documents/facts/ictfacts Figures2015.pdf
3. Yakymenko I. Long-term exposure to microwave radiation provokes cancer growth: evidences from radars and mobile communication systems / I. Yakymenko, E. Sidorik, S. Kyrylenko,V. Chekhun // Exp Oncol. – 2011. – V. 33. – P. 62–70.
4. Yakymenko I. Low intensity radiofrequency radiation: a new oxidant for living cells / I. Yakymenko, E. Sidorik, D. Henshel, S. Kyrylenko // Oxid Antioxid Med Sci. – 2014. – V. 3. – P. 1–3.
5. Draper H. H. Malondialdehyde determination as index of lipid peroxidation / H. H. Draper, M. Hadley // Methods in enzymology. – 1990. – V. 186. – P. 421–31.
6. Королюк М. А. Метод определения активности каталазы / М. А. Королюк, Л. И. Иванова, И. Г. Майорова, В. Е. Токарев // Лаб. дело. – 1988. – V. – P. 16–19.
7. Чавари С. Роль супероксиддисмутазы в окислительных процессах клетки и метод определения ее в биологических материалах / С. Чавари, И. Чаба, Й. Секуй // Лаб. дело. – 1985. – V 39. – P. 678–681.
8. Тен Э. В. Экспресс-метод определения содерæания церулоплазмина в сыворотке крови / Э. В. Тен // Лаб. дело. – 1981. – V. – P. 334–335.
9. Valko M. Free radicals and antioxidants in normal physiological functions and human disease / M. Valko, D. Leibfritz, J. Moncol, M. T. Cronin, M. Mazur, J. Telser // The international journal of biochemistry & cell biology. – 2007. – V. 39. – P. 44–84.
10. Valko M. Free radicals, metals and antioxidants in oxidative stress-induced cancer / M. Valko, C. J. Rhodes, J. Moncol, M. Izakovic, M. Mazur // Chemico-biological interactions. – 2006. – V. 160. – P. 1–40.
11. Burlaka A. Overproduction of free radical species in embryonal cells exposed to low intensity radiofrequency radiation / A. Burlaka, O. Tsybulin, E. Sidorik, S. Lukin, V. Polishuk, S. Tsehmistrenko,I. Yakymenko // Exp Oncol. – 2013. – V. 35. – P. 219–225.
12. Nguyen H. L. Oxidative stress and prostate cancer progression are elicited by membrane-type 1 matrix metalloproteinase / H. L. Nguyen, S. Zucker, K. Zarrabi, P. Kadam, C. Schmidt, J. Cao // Mol Cancer Res. – 2011. – V. 9. – P. 1305–1318.
13. Ralph S. J. The causes of cancer revisited: «Mitochondrial malignancy» and ROS-induced oncogenic transformation – Why mitochondria are targets for cancer therapy / S. J. Ralph, S. Rodríguez-Enríquez, J. Neuzil et al. // Molecular aspects of medicine. – 2010. – V. 31. – P. 145–170.
14. Forman H. J. An overview of mechanisms of redox signaling / H. J. Forman, F. Ursini,M. Maiorino // Journal of molecular and cellular cardiology. – 2014. – V. 73. – P. 2–9.
15. Sies H. Role of metabolic H2O2 generation: redox signaling and oxidative stress / H. Sies // The Journal of biological chemistry. – 2014. – V. 289. – P. 8735–8741.
16. Oshino N. Optical measurement of the catalase-hydrogen peroxide intermediate (Compound I) in the liver of anaesthetized rats and its implication to hydrogen peroxide production in situ / N. Oshino, D. Jamieson, T. Sugano, B. Chance // The Biochemical journal. – 1975. – V. 146. – P. 67–77.
17. Enyedi B. H2O2: a chemoattractant? / B. Enyedi, P. Niethammer // Methods in enzymology. – 2013. – V. 528. – P. 237–255.
18. Hayden M. S. NF-kappaB in immunobiology / M. S. Hayden,S. Ghosh // Cell research. – 2011. – V. 21. – P. 223–244.
19. Tsybulin O. GSM 900 MHz microwave radiation affects embryo development of Japanese quails / O. Tsybulin, E. Sidorik, S. Kyrylenko et al. // Electromagnetic biology and medicine. – 2012. – V. 31. – P. 75–86.
20. Tsybulin O. GSM 900 MHz cellular phone radiation can either stimulate or depress early embryogenesis in Japanese quails depending on the duration of exposure / O. Tsybulin, E. Sidorik, O. Brieieva et al. // International journal of radiation biology. – 2013. – V. 89. – P. 756–763.
21. Calabrese E. J. Hormesis: why it is important to toxicology and toxicologists / E. J. Calabrese // Environ Toxicol Chem. – 2008. – V. 27. – P. 1451–1474.

Published

2016-12-17

How to Cite

Tsybulin О. (2016). Influence of Microwave Radiation on Antioxidant System in the Tissues of Embryos and Daily Quail. Notes in Current Biology, (12(337), 126–131. https://doi.org/10.29038/2617-4723-2016-337-12-126-131