The influence of presowing treatment on the content of lipid oxidation products, vitamins and the activity of antioxidant enzymes in winter rye grain

Authors

  • Anton Kurylenko Nizhyn Gogol State University
  • Olena Kuchmenko Nizhyn Gogol State University

DOI:

https://doi.org/10.29038/2617-4723-2022-1-1-3

Keywords:

winter rye, presowing treatment, vitamin E, ubiquinone-10, peraoxybenzoic acid, methionine, MgSO4, vitamins, antioxidant enzymes, lipid oxidation products

Abstract

Winter rye is a promising crop for the Polissya region of Ukraine, so the search for and development of effective and safe approaches to stimulate the growth and development of this crop is relevant. The material of the study was the seeds of winter rye (Secale cereale L.) varieties Synthetic 38 and Zabava, and compositions of metabolically active substances: vitamin E (10 -8 M), peraoxybenzoic acid (POBA) (0,001%), methionine (0,001%), ubiquinone-10 (10 -8 M) and MgSO4 (0,001%), which were used in the following compositions – vitamin E+POBA+methionine, vitamin E+POBA+methionine+MgSO4 , vitamin E+ubiquinone-10. For the first time, presowing treatment of seeds with the studied compositions was performed and research was carried out on the content of products of free radical oxidation of lipids, antioxidant vitamins, and activity of antioxidant enzymes in the grain of winter rye varieties Syntetyk 38 and Zabava. The effectiveness of vitamin E+POBA+methionine+MgSO4 and vitamin E+ubiquinone-10 compositions in increasing the content of low-molecular-weight antioxidant vitamins (ascorbic acid and carotenoids), reducing the activity of antioxidant enzymes (catalase and ascorbate peroxidase) and the content of lipid free radical oxidation products was demonstrated in winter rye grains of the Sintetyk 38 and Zabava varieties. The vitamin E+POBA+methionine+MgSO4 composition demonstrated the most effectiveness in this regard. The demonstrated increase in the content of antioxidants in winter rye grain can increase the nutritional value of products made from this grain. In addition, the demonstrated changes in the activity of the components of the pro- and antioxidant systems may indicate that the grain is more deeply in a state of rest after presowing seed treatment with the studied compositions.

References

Kunah, O. M.; Pakhomov, O. Y.; Zymaroieva, А. А.; Demchuk, N. I.; Skupskyi, R. M.; Bezuhla, L. S.; Vladyka, Y. P. Agroeconomical and agroecological aspects of the rye (Secale cereale L.) yelds spatial variation within Polesia and Foreststeppe zones of Ukraine: the useage of the geographically weighted principal components analysis. Biosystems Diversity. 2018, 26(4), pp 276–285.

Kulichova, K.; Sokol, J.; Nemecek, P.; Maliarova, M.; Maliar, T.; Havlentova, M.; Kraic, J. Phenolic compounds and biological activities of rye

(Secalecereale L.) grains. Open Chem. 2019, 17, pp 988–999.

Dynkowska, W.M. Rye (Secalecereale L.) phenolic compounds as health-related factors. Plant breeding and seed science. 2019, 79, p 9–24.

Linina, A.; Augspole, I.; Romanova, I.; Kuzel, S. Winter rye (Secale cereale L.) antioxidant capacity, total phenolic content and quality indices. Agronomy Research. 2020, 18(S3), pp 1751–1759.

Gumul, D.; Berski, W. The polyphenol profile and antioxidant potential of irradiated rye grains. International Journal of Food Science. 2021, article ID 8870754, 7 pages.

Kaur, P.; Sandhu, K. S.; Bangar, S. P.; Purewal, S. S.; Kaur, M.; Ilyas, R. A.; Asyraf, M.R.M.; Razman, M. R. Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (Secale cereale) flour. Antioxidant. 2021, 10, pp 1214–1228.

Jiang, K.; Asami, T. Chemical regulators of plant hormones and their applications in basic research and agriculture. Bioscience, Biotechnology, and Biochemistry. 2018, 82(8), pp 1265–1300.

Horobets, M.; Chaika, T.; Krykunova, V. Influence of growth stimulants on the ontogenesis of spring barley (Hordeum Vulgare L.). Colloquium-journal. 2021, 7(94), pp 41–42.

Sumanta, N.; Haque, C. I.; Nishika, J.; Suprakash, R. Spectrophotometric analysis of chlorophylls and carotenoids from commonly grown fern species by using various extracting solvents. Res. J. Chem. Sci. 2014, 4, pp 63–69.

Hasanuzzaman, M.; Nahar, K.; Alam, M.; Fujita, M. Exogenous nitric oxide alleviates high temperature induced oxidative stress in wheat (Triticum aestivum L.) seedlings by modulating the antioxidant defense and glyoxalase system. Aust. J. Crop Sci. 2012, 6, pp 1314–1323.

Alam, M.; Nahar, K.; Hasanuzzaman, M.; Fujita, M. Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species. Plant Biotechnol. 2014, 8, pp 279–293.

Nakano, Y.; Asada, K. Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts. Plant Cell Physiol. 1981, 22, pp 867–880.

Aebi, H. Catalasein Vitro. Methods Enzymol. 1984, 105, pp 121–126.

Yakhin, O. I.; Lubyanov, A. A.; Yakhin, I. A.; Brown, P. H. Biostimulants in plantscience: A global perspective. Frontiers in Plant Science. 2017, 7, p 2049.

Korotkova, I. V., Horobets, M. V., Chaika, T. O. Vplyv Stymuliatoriv Rostu Na Produktyvnist Sortiv Yachmeniu Yaroho [Influence of Growth Stimulators on Productivity of Spring Barley Varieties]. Visnyk Poltavskoi Derzhavnoi Ahrarnoi Akademii. 2021, 2, 20–30. (in Ukrainian)

Wang, X.; Liu, H.; Yu, F.; Hu, B.; Jia, Y.; Sha, H.; Zhao, H. Differential activity of the antioxidant defence system and alterations in the accumulation of osmolyte and reactive oxygen species under drought stress and recovery in rice (Oryza sativa L.) tillering. Sci. Rep. 2019, 9, p 8543.

Kurylenko, A. O.; Kurylenko, O. V.; Kuchmenko, O. B.; Havii, V. M. Vplyv peredposivnoi obrobky nasinnia kompozytsiiamy metabolichno aktyvnykh rechovyn na morfometrychni pokaznyky ozymoho zhyta v umovakh Polissia Ukrainy [The influence of pre-sowing treatment of seed swith the composition of metabolically active substances on the morphometric indicators of winter ryein the conditions of Polissia of Ukraine]. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia: Ahronomiia i biolohiia. 2021, 4, 25–32. (in Ukrainian)

Skrypnik, L.; Maslennikov, P.; Novikova, A.; Kozhikin, M. Effect of Crude Oil on Growth, Oxidative Stress and Response of Antioxidative System of Two Rye (Secale cereale L.) Varieties. Plants. 2021, 10(1), p 157.

Published

2022-06-30

How to Cite

Kurylenko А., & Kuchmenko О. (2022). The influence of presowing treatment on the content of lipid oxidation products, vitamins and the activity of antioxidant enzymes in winter rye grain. Notes in Current Biology, (1 (1), 18–22. https://doi.org/10.29038/2617-4723-2022-1-1-3