Cultivation of Sparassis laminosa on plant substrates

Authors

DOI:

https://doi.org/10.29038/NCBio.21.1.9-14

Keywords:

fruiting bodies, Sparassis laminosa 2211, re-situ

Abstract

Sparassis laminosa is an edible species, has pharmaceutically value and can be used for cultiva-tion. The fungus is rare in Belarus, in some regions of the Russian Federation and in Poland. In Ukraine, this species is proposed to be included in the "List of species that are endangered and subject to protection." Fungus has the large size of the fruiting bodies up to 60 cm in diameter and weighing up to 9 kg. It is easy to detect and remove from nature that increases the risk of extinction of the species.
The aim of the study was to detect conditions of S laminosa fruiting on plant substrates, modified by leaf and the prospects of the modified substrates for industrial cultivation f the species.
The studies were performed with a pure culture of S. laminosa 2211 in three stages. In the first stage, the culture was grown for 14 days in the Petri dishes on WA. In the second stage, the mycelium from the Petri dish was transferred in a 0.5 liter glass jars. Jars contained plant substrates such as wheat grains, sunflower husks, coniferous shavings, wheat straws (in the ratio 8: 2: 1: 1). Mycelium overgrew plant substrate at 15 days. In the third stage, the mycelium together with the plant substrate was placed in the sterilization boxes with sterile fallen leaves.
It was found that complete fouling of the leaves by the mycelium of the fungus takes place on 40 ± 2 days of the experiment, and the first fruiting bodies of S. laminosa appear at 46 days after the adding of the plant sub-strate to the leaves. Fruiting bodies have a typical type of morphology, cream color, are formed in places of light access, in the amount of one fruit body per box. Formed fruiting bodies not exceeding 15-20 × 10-15 cm, weight not more than 70 g, so the technique needs refinement. We should change the container where fruiting is recorded. Need a container that would maintain a stable humidity of the substrate during cultivation and improving the light regime. These changes will allow adjusting the conditions for fruiting of S. laminosa and reach acceptable for in-dustry parameters.
The obtained results are important for their application in the implementation of the re-situ technique. Using fallen leaves and overgrowth them by mycelium before its removal into nature will obviously reduce the process of adaptation of the mycelium to natural conditions and thus increase its viability.

References

1. Kryvko N. N.; Bondarenko E. N. Podbor sred y temperaturnoho rezhyma dlia vyrashchyvanyia hryba Sparassis laminosa v kulture. [Selection of media and temperature conditions for growing Sparassis laminosa in culture]. Mykolohyia y alholohyia. Materyaly yubyleinoi konferentsyy posviashchennoi 85-Letyiu kafedry Mykolohyy y Alholohyy MHU Ym. M.V. Lomonosova. Moskva, 2004. s. 79‒80. (in Russian)
2. Mudryk, E. A.; Novikova, V. V.; Suhomlin, M. N. Poisk novyh vidov gribov dlja kul`tivirovanija. Sparassis laminosa (Fr.) [Selection the new species for cultivation. Sparassis laminose (Fr.)]. Tezi Mіzhnarodnoi` konferencіi` ”Problemi suchasnoi` ekologіi`”, Zaporіzhzhja, 20-22 veresnja, 2000 r. s. 107. (in Russian)
3. Krasnaja kniga respubliki Belarus` (jelektronnyj resurs). [Red book of Belarus]. Website: URL: http://redbook.minpriroda.gov.by vіd 2006. (in Russian)
4. Spisok rastenij, zhivotnyh, gribov i lishajnikov, zanesennyh v Krasnuju knigu Lipeckoj oblasti. 2021. (jelektronnyj resurs). [List of plants, animals, fungi and lichens included to Red book of Lipetsk]. Website: URL:http://ekolip.ru/oopt/spisok-kk/?SECTION_ID=247&ELEMENT_ID=1078 (in Russian)
5. Wojewoda, W., Ławrynowicz, M., 2006 — Czerwona lista grzybów wielkoowocnikowych w Polsce. Website. URL: https://grzyby.pl/czerwona-lista-grzybow.htm (in Polska)
6. Leshan, T. A.; Pakhomov O. Ye. Rarytetnyi fond bazydiomitsetiv Skhodu Ukrainy [Rare fund of Basidiomycetes of East of Ukraine]. Visnyk Dnipropetrovskoho Universytetu. Biolohiia. Ekolohiia 2009, vyp. 17 (1). s. 115–120. (in Ukrainian)
7. Zerova, M. Ya.; Radziievskyi H. H.; Shevchenko S. V. Vyznachnyk hrybiv Ukrainy. Tom 5. Knyha 1. - Bazydiomitsety Ekzobazydialni Afiloforalni Kantarelalni. [Determinant of fungi of Ukraine. Volume 5. Book 1. - Basidiomycetes: Exobasidiales, Aphilophorales, Cantharellales]. Kyiv: Naukova Dumka, 1972, 240 s. (in Ukrainian)
8. Vynaev, G. V.; Gapienko, O. S. Sparassis plastinchatyj (Sparassis laminosa Fr.) — novyj vid afilloforovyh gribov (Aphyllophorales) dlja mikobioty Belarusi. [Lamellar sparassis (Sparassis laminosa Fr.) is a new species of aphyllophorales (Aphyllophorales) for the mycobiota of Belarus]. Botanicheskie sady: sostojanie i perspektivy sohranenija, izuchenija, ispol`zovanija biologicheskogo raznoobrazija rastitel`nogo mira: Tezisy dokladov Mezhdunarodnoj nauchnoj konferencii. Minsk, 30 – 31 maja 2002, s. 48–49. (in Russian)
9. Sukhomlyn M. M. Problema sumisnosti u vyshchykh Bazydiomitsetiv (Fiziolohichni ekolohichni morfolohichni aspekty) [The problem of compatibility in higher basidiomycetes (physiological, ecological, morphological aspects)]. Dys.... D-ra biol. nauk - K. 2003. 269 S.
10. Berezovska, M.; Pavlovska, M.; Karbovska, V.; Karpenko, N.; Abduloeva, O.; Kondratiuk, T.; Sukhomlyn, M.; Kostikov, I. Znachennia kolektsii u zberezhenni bioriznomanittia u suchasnii naukovii diialnosti. [Collection’s value for saving of biodiversity in the modern scientist action]. Visnyk Kyivskoho natsionalnoho universytetu. Problemy rehuliatsii fiziolohichnykh funktsii. 2012, vyp. 15. s. 44-47. (in Ukraine)
11. Bisko, N. A.; Sukhomlyn, M. M.; Mykchaylova, O. B.; Lomberg, M. L.; Tsvyd, N. V.; Petrichuk, Yu. V.; Al-Maali, G. A.; Mytropolska, N. Yu. Ex situ conservation of rare and endangered species in mushroom culture collections of Ukraine. Ukrainian Botanical Journal 2018, 75(4). c 338–347. https://doi.org/10.15407/ukrbotj75.04.338
12. Bisko, N. A.; Lomberg, M. L.; Mytropolska, N. Y.; Mykchaylova, O. B. The IBK mushroom culture collection. Alterpres: Kyiv, 2016; 120 s.
13. Sukhomlyn, M. M. Nakopychennia metaliv vyshchymy Bazydialnymy hrybamy. [Accumulation of metals by higher basidiomycetes]. Pytannia Bioindykatsii ta Ekolohii 2000, vyp. 5(3)., s. 199-205. (in Ukrainian)
14. Sukhomlyn, M. M. Skryninh Hrybiv-Antahonistiv Korenevoi Hubky. [Screening of fungi antagonists of the root sponge]. Naukovyi visnyk Natsionalnoho ahrarnoho universytetu. Lisivnytstvo. 2000. № 27. S 296-299. (in Ukrainian)
15. Yarosh R. M.; Lomberh M. L.; Krasinko V. O. Vyroshchuvannia hrybiv z likuvalnymy vlastyvostiamy na riznykh substratakh. [Growing mushrooms with medicinal properties on different substrates]. Materialy VI Mizhnarodnoi naukovo-praktychnoi konferentsii «Biotekhnolohiia zvernennia ta nadii» prysviachenoi 120-Richchiu NUBiP Ukrainy. Kyiv 14-16 lystopada, 2017, s. 251‒253. (in Ukrainian)
16. Tsvyd N. V.; Petrychuk Yu. V.; Sukhomlyn M. M. Ridkisni vydy Afiloforoidnykh hrybiv (Sparassis laminosa ta Hericium cirrhatum) v umovakh chystoi kultury. [Rare species of Aphillophorales fungi (Sparassis laminosa and Hericium cirrhatum) in pure culture]. Materialy IV Mizhnarodnoi konferentsii «Ridkisni roslyny i hryby Ukrainy ta prylehlykh terytorii realizatsiia pryrodookhoronnykh stratehii». Palyvoda Kyiv 16 – 20 Travnia, 2016, s. 199–201. (in Ukraininan)
17. Pasailiuk, M.; Sukhomlyn, M.; Gryganskyi, A. Biological features of Sparassis laminosa Fr. (Sparassidaceae, Polyporales) and the main aspects of its reproduction in the territory of Hutsulshchyna National Natural Park, Ukraine. Current Research in Environmental & Applied Mycology (Journal of Fungal Biology), 2019, 9(1), рр. 194–207. DOI:10.5943/cream/9/1/17
18. Pasailiuk, M. Growing of Polyporus umbellatus. Current Research in Environmental & Applied Mycology (Journal of Fungal Biology), 2020, 10(1), рр. 457–465. DOI: 10.5943/cream/10/1/35
19. Sung-Ryul, R.; Kang-Hyeon, K.; Hyun P.; Won-Chull B.; Bong-Hun L. Cultivation Characteristics of Sparassis crispa Strains Using Sawdust Medium of Larix kaempferi. The Korean Journal of Mycology, 2009, 37(1), рр. 49-54.
20. Dezhi, L.; Jong-Soo, K. Sparassis crispa cultivation method. Patent CN103650913A. China2013.

Published

2021-09-13

How to Cite

Cultivation of Sparassis laminosa on plant substrates. (2021). Notes in Current Biology, 1 (1), 9-14. https://doi.org/10.29038/NCBio.21.1.9-14